okay one of the tissue culture methods detection is PLAQUE ASSAY. What is it and how does it work? Let's find out together, shall we?
Plaque Assay:
A method of quantifying the number of infectious units by inoculating serial dilutions of a viral suspension on a cell culture monolayer, overlaying with a medium containing agarose and after several days incubation, counting the number of plaques formed; recorded as plaque forming units/ml. These plaques can sometimes be detected visually using colony counters, in much the same way as bacterial colonies are counted. This technique requires counting the number of plaques formed by a virus sample, from which the actual virus concentration can be determined.
Features:
- Very time-consuming
- Very simple method
- Only works for viruses that infect monolayer cells
- Only works for viruses that causes cell lysis
- Uses principle of one virus on the monolayer produces one plaque
To know the steps to do Plaque Assay, please watch this video :
Is it more clearer now? We hope you understood plaque assay better. Stay tuned again! We will be updating on the second method which is Cytopathic Effect (CPE).
till then.
:D
-fatin(:
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